|Biomedical and Pharmaceutical Science Course
Laboratory of Structural Biology
(Fuyuhiko Inagaki's Lab)
|Structural Biology of Intracellular Signal Transduction|
|Structural Biology, NMR, X-ray crystallography, signal transduction network, domain, adaptor protein, scaffold protein, neutrophil NADPH oxidase, interferon generating system, autophagy|
| @Multicellular organisms form organized cell society by communicating with each other. For this purpose, the cells develop highly complex signal transduction network. The signals mediated by cell surface receptors are transmitted into the cell, diverge in the cytoplasm, crosstalk and are finally integrated in the nucleus to induce gene expression essential for cell-response. Due to the signal transduction network, the organisms can respond flexibly to the external stimuli and smoothly exert biological functions. Scaffold proteins and adaptor proteins play critical roles in signal transduction network. These proteins are comprised of multiple functional domains but have no enzymatic activities and link upper and downstream signals. Our major interest is to elucidate the structural basis of these proteins in regulation of signal transduction.
@Our laboratory was established in 1999 for studying structural biology of intracellular signal transduction by NMR and X-ray crystallography. Our research effort in recent years concentrates on the following five subjects: 1) Structural and functional analyses of novel domains involved in scaffold and adaptor proteins; 2) Studies on the structural basis of the regulatory mechanism mediated by domain-domain interaction; 3) Structural biology of innate immunity that focuses on the regulation of neutrophil super oxide generating system and the signaling network responsible for production of type I interferon; 4)Structural biology of autophagy as a cell-response to starvation signal; 5) Development of Olivia, a platform for high throughput analysis of NMR protein structure determination.