Biomembranes are essential for multiple cellular activities including cell shape, cell
polarity, vesicle transport, and organelle functions. Because biomembranes consist of lipid bilayers which are composed of various lipid molecules in an asymmetrical manner between bilayers; lipids are believed to play an important role for membrane
functions. However, there is still so much which remains to be learned, because lipids are elusive small molecules. The aim of our laboratory is to elucidate the roles of lipid dynamics in various cellular functions by using yeast as a model organism.

Plants are commonly considered as inanimate and “still life” objects, however, we are simply limited in our abilities to directly sense their movement in our temporal sense. Upon close evaluation with time-lapsed photography, it becomes evident that plants are very animated and their dynamic movement and development is revealed. The plant hormone “Auxin” has a central functional role in plant morphogenesis. The main focus of this laboratory pertains to the study of “Early-Auxin Inducible Genes” and to investigate the functional roles of auxin in the synthesis of new organs (Organogenesis). We primarily utilize Arabidopsis as a functional model system for both forward and reverse genetics. In addition, we also use both potato and carrot as additional models.

Plants possess multiple attractive features and potential that we, as humans, simply lack. For example, plants are readily capable to regenerate an entire organism from a single cell by using only light, water and micronutrients. In addition, stem cells for most plant species possess capabilities for indefinite cell proliferation. Plants are capable of surviving exposure to dynamic seasonal conditions from summer to winter. While plants are actively photosynthesizing, plants will never die from “cancer-like” effects even in continuous exposure to sunlight. Over the course of millions of years, plants have evolved many unique and beneficial characteristics which have the potential to bring great value for human beings and our mother earth. One of our primary goals is to unveil these powerful attributes of plants or plant cells and to learn their underlying mechanisms. As a result, these findings may enable us to develop novel plants which are capable of enhanced growth and productivity even under harsh environmental conditions.

Plants acquire highly diverse morphologies during evolution. This diversity is a consequence of the plant's unique principle of growth and development, which is governed by a genetic program, but is modulated by environmental signals. Polar auxin transport, which is controlled by the polar localization of the auxin efflux carrier PIN protein, acts as the basis for this principle. The polar localization of PIN is regulated by various cellular factors, including membrane trafficking, membrane fluidity and cytoskeleton. Moreover, PIN localization is also regulated by environmental signals, including light and gravity signals, as well as the starvation and salinity stress. Because of this complexity, the mechanisms that regulates the polar localization of PIN remain elusive. We introduce cellular and molecular biological techniques to elucidate the mechanisms of polar localization of PIN. Employing mathematical and theoretical biological techniques, we elucidate the relationship between the evolution of the molecular basis of PIN localization and the morphological diversification during plant evolution. In our experiments, variety of plants, including seed plants, ferns, mosses and algae will be used.

For many years, DNA was known as the key cellular component which captured encoded genetic information and served as the template for active cellular components; beginning with RNA and subsequently functional proteins. However, active RNA molecules and epigenetic modifications have been recently shown to play critical and highly specific regulatory roles for important biological activities. By using plants as our model system, we study the functional mechanisms of non-coding RNAs and epigenetic regulation. We are very interested to understand how the effects of the epigenetic regulation impact the capability of plants to adapt to their environment. A main objective of our studies is to identify novel gene regulation factors. Secondly, we also aim to develop enhanced plants through application of the identified regulation factors.

Plant growth and development are controlled by the concerted actions of many
signaling pathways which are triggered by developmental and metabolic cues.
Nutrients such as sugars and nitrogen compounds play an important role as
signaling molecules that modulate the expression of many plant genes involved in
diverse physiological processes, such as germination, seedling development,
flowering, senescence, and pathogen responses. Our goal aims to clarify
signaling networks which mediate nutrient utilization and plant growth regulation.

The control of gene expression is exerted by multiple steps such as transcription, RNA
processing and export, mRNA stability, translation and post-translational events. The recent discovery of biologically active small RNAs has enhanced the impact of post-transcriptional regulation, and in particular, the alterations of mRNA stability on gene regulation. The control of mRNA turnover is thought to be an important component of the rapid response to environmental changes. The goal of our projects is to demonstrate the importance of mRNA turnover control in regulating gene expression in response to several stress conditions in plants.

Plants in nature are exposed to a massive variety of microbes. Just like human health relies on the appropriate coordination of gut microflora, plant physiology is immensely influenced by its associated microbial community (the plant microbiota). Our group focuses on the molecular dialog between plants and microbiota and aims to understand the molecular mechanisms by which plant microbiota interfere with plant development and immunity. Our ultimate goal is to describe the true art of life of plants in nature at the molecular level by exploring plant-microbe interaction and its evolution.

When animals receive sensory stimuli from environment, they perform appropriate behaviors via sensory perception, decision making, and motor planning that are mediated by their central nervous system. By using a combination of behavioral analyses, electrophysiology, optical imaging and machine-learning approaches, we address understanding the neural basis underlying wind-elicited behavior in the cricket as a behavioral model. The ultimate goal of our research is a complete understanding of whole neural circuitry and computational process from sensory perception to motor output in specific behaviors.

Our research incorporates both mechanistic and functional approaches to address the question why living things show such a great diversity in behaviors and their underlying cognitive functions. We focus primarily on functional aspects and we investigate the behavioral ecology of passerine birds (mainly Estrildid finches) with particular interest in social communication; including courtships and parent-offspring interactions,
reproduction, development and life history.

Animal behavior is influenced by both environmental and genetic factors. However, much remains to be learned regarding how and when the environmental and genetic
factors act and how developing behavior itself affects the molecular basis in the neuronal substitute. The primary focus of our laboratory is to elucidate these questions by using songbirds as an animal model for vocal learning and to identify its critical period.

Our goal is to reveal (i) how neuronal circuits work as a system to process sensory inputs, (ii) how this processing is modulated by a specific environment or by the mental state or the condition of the body, (iii) how behavioral decisions are made according to previous experiences and the current situation, and (iv) how these processes depend on the dopaminergic and octopaminergic systems. The answers to these questions will reveal how an animal displays adaptive behavior in response to a given situation that changes every moment or even develops intelligent behavior to cope with a difficult situation, and also help us invent biologically inspired sensors and environmental-friendly pest control. To study these problems in our laboratories, we are utilizing invertebrate animals such as insects and cephalopods.

Humans use only one hand for performing most tasks in various situations. Such lateral preference in behaviors has been widely documented in many vertebrates and invertebrates. The dominant side may have possibly better locomotion performance (accuracy and/or speed) than the non-dominant side. However, the differences in the nervous systems between righty and lefty, the developmental process of dominance, what genes and molecules regulate dominance, and when it was established evolutionarily still remain essential mysteries. Using African scale-eating cichlids, which are known for their pronounced handedness, I am investigating the mechanisms governing laterality from multiple approaches.

If we sleep eight hours a day, we spend as much as one-third of our lives sleeping. Why do we sleep? Why do we dream? Why do we have rapid eye movement (REM) and non-REM sleep? We are still unable to correctly answer such fundamental questions. In our laboratory, we perform research to answer these questions using genetically engineered mice. We aim to clarify the neural mechanisms and physiological functions of dreams, in particular.

The supply of germ cells (oocytes) to produce the next generation is the most important function of the ovary. We are studying the unsolved issues associated with ovarian functions. We are analyzing to elucidate the mechanism of oogenesis, ovulation, and tissue repair after ovulation using mouse, medaka, and zebrafish.

Steroid hormones regulate physiological responses in vertebrates by binding to the nuclear receptors (NR), a ligand-activated transcription factor. We study the evolution of vertebrate NRs and investigate how steroid hormones act in many vertebrates. We also analyze the temperature-dependent sex-determination/ differentiation of reptiles, and the effects of endocrine disrupting chemicals; including the evaluations of pharmaceuticals on animals.

How is sex determined in animals? We are interested to study sex determination, regulation of chromatin, fertilization in both birds and mammals. We perform functional analysis of various genes of domestic birds such as chicken, quail and emu by using transgenic, genome editing or biotechnological methods. We also aim to identify a novel sex determination gene and to reveal a process of Y-loss in the genus Tokudaia which lacks a Y chromosome.

Most of genome sequences are nongenic regions that regulate gene expression and are transcribed as noncoding RNAs. We aim to elucidate the physiological significance and molecular mechanism of genome sequences in reproduction. Our main projects are multifunctional genome elements in testis, long noncoding RNAs in testis and ovary, and the evolution of regulation of oogenesis.

A multicellular organism begins with a single cell, the fertilized egg, and subsequently undergoes differentiation and morphogenesis to become an embryo. All cellular functions and patterning decisions that occur prior to the activation of the zygotic genome depend on maternal factors deposited in the egg during oogenesis, which become active after fertilization. By using zebrafish and mouse as model systems, we investigate the deposition of maternal factors in oocytes that promote developmental processes. Our aim is to identify novel mechanisms of oogenesis and developmental processes by isolating and analyzing maternal factors.

Completion of the genome sequence project and development of next-
generation sequencing technique have dramatically changed a design of
research strategy in biomedical science. However, functions of all genes in the
whole genome are not well understood even now. We elucidate functions of
disease-related genes by use of gene-manipulated rats and mice generated in
our laboratory.